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Related Concept Videos

Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
Chromosome Replication02:31

Chromosome Replication

Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin of...

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Updated: Jun 27, 2026

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG
10:11

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG

Published on: July 26, 2024

Structural basis for DNA binding by replication initiator Mcm10.

Eric M Warren1, Sivaraja Vaithiyalingam, Justin Haworth

  • 1Department of Biological Sciences, Vanderbilt University, Nashville, TN 37232, USA.

Structure (London, England : 1993)
|December 17, 2008
PubMed
Summary
This summary is machine-generated.

Mcm10, a key DNA replication protein, uses a unique zinc finger to bind single-stranded DNA. This interaction is crucial for replication and preventing stress in cells.

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Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG

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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Biochemistry

Background:

  • Mcm10 is vital for eukaryotic DNA replication fork assembly and progression.
  • Mcm10's internal domain (Mcm10-ID) interacts with single-stranded DNA, DNA polymerase alpha, and PCNA.

Purpose of the Study:

  • To determine the crystal structure of Xenopus laevis Mcm10-ID.
  • To elucidate the mechanism of Mcm10's single-stranded DNA binding.
  • To investigate the functional significance of Mcm10 DNA binding in vivo.

Main Methods:

  • X-ray crystallography to determine the Mcm10-ID structure.
  • NMR chemical shift perturbation and in vitro mutagenesis to study DNA binding.
  • Analysis of Mcm10 mutations in Saccharomyces cerevisiae to assess replication stress sensitivity.

Main Results:

  • The crystal structure reveals Mcm10-ID possesses an oligonucleotide/oligosaccharide-fold followed by a basic zinc finger.
  • NMR and mutagenesis studies demonstrate this region directly binds single-stranded DNA.
  • Mutations affecting this DNA binding in yeast increase sensitivity to replication stress.

Conclusions:

  • The Mcm10 zinc finger is essential for direct single-stranded DNA binding.
  • This DNA binding function is critical for cellular replication fidelity and stress response.
  • The structure provides a framework for understanding Mcm10's role in coordinating replisome components.