Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Somatic Mutation Profiles in Colorectal Cancers Differ by Population.

medRxiv : the preprint server for health sciences·2026
Same author

Race and Socioeconomic Status Impact Survival from Early and Late-Onset Colorectal Cancer.

medRxiv : the preprint server for health sciences·2026
Same author

Non-Invasive Evaluation of Pulmonary Artery Stenosis in Congenital Heart Disease Patients Using Phase Contrast MRI.

Cardiovascular engineering and technology·2026
Same author

Diagnostic Cutoff Values of Aortic Valve Coefficient (AVC) in Relation to Aortic-Valve Area and Calcium Score: A Patient-Level Analysis.

Structural heart : the journal of the Heart Team·2026
Same author

Diagnostic Cut-off Values for Newly Developed Noninvasive Elastance-Based Indices in Duchenne Muscular Dystrophy-Associated Cardiomyopathy.

Pediatric cardiology·2026
Same author

Miniaturized volatile organic compound sensor platform for the real-time monitoring of bacterial growth and antibiotic susceptibility.

Analytica chimica acta·2026

Related Experiment Video

Updated: Jun 27, 2026

In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity
10:48

In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity

Published on: December 17, 2017

Effect of simulated microgravity on oxidation-sensitive gene expression in PC12 cells.

Ohwon Kwon1, Maureen Sartor, Craig R Tomlinson

  • 1Department of Mechanical Engineering, University of Cincinnati, Cincinnati, OH 45221, USA.

Advances in Space Research : the Official Journal of the Committee on Space Research (COSPAR)
|December 17, 2008
PubMed
Summary

Cellular processes change in microgravity, affecting oxygen use. This study found over 100 genes, particularly those in oxidoreductase activity, were altered in rat pheochromocytoma (PC12) cells under simulated microgravity.

More Related Videos

Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System
09:28

Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System

Published on: August 25, 2022

Induction and Analysis of Oxidative Stress in Sleeping Beauty Transposon-Transfected Human Retinal Pigment Epithelial Cells
10:00

Induction and Analysis of Oxidative Stress in Sleeping Beauty Transposon-Transfected Human Retinal Pigment Epithelial Cells

Published on: December 11, 2020

Related Experiment Videos

Last Updated: Jun 27, 2026

In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity
10:48

In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity

Published on: December 17, 2017

Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System
09:28

Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System

Published on: August 25, 2022

Induction and Analysis of Oxidative Stress in Sleeping Beauty Transposon-Transfected Human Retinal Pigment Epithelial Cells
10:00

Induction and Analysis of Oxidative Stress in Sleeping Beauty Transposon-Transfected Human Retinal Pigment Epithelial Cells

Published on: December 11, 2020

Area of Science:

  • Space Biology
  • Cellular and Molecular Biology

Background:

  • Cellular oxygen utilization and dependence may differ in microgravity.
  • Understanding these changes is crucial for baseline cellular responses in space.
  • Oxidation-sensitive molecular processes are key areas of investigation.

Purpose of the Study:

  • To analyze oxidation-sensitive gene expression in a model cell line under simulated microgravity.
  • To establish a baseline for cellular changes in microgravity conditions.
  • To investigate the impact of microgravity on oxygen-sensitive molecular pathways.

Main Methods:

  • Utilized the rotating wall vessel (RWV) bioreactor to simulate microgravity.
  • Employed rat pheochromocytoma (PC12) cells, a well-characterized model for oxygen response.
  • Analyzed gene expression using DNA microarray technology.

Main Results:

  • Over 100 genes showed at least twofold expression changes (65 upregulated, 39 downregulated) in simulated microgravity compared to unit gravity.
  • Genes involved in oxidoreductase activity were significantly differentially expressed.
  • Key oxidation-sensitive transcription factors, including HIF-2α, c-myc, and PPAR-γ, showed significant changes.

Conclusions:

  • Simulated microgravity significantly alters gene expression in PC12 cells, particularly affecting oxidoreductase activity.
  • These findings provide a foundation for evaluating oxygen's role in microgravity-induced gene regulation.
  • Further research can explore variable oxygen environments on differential gene regulation under altered gravity.