Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Production of Alcohol01:27

Production of Alcohol

Continuous fermentation is a key strategy in industrial ethanol production, particularly when efficiency, scalability, and high yields are essential. This approach allows for uninterrupted operation and optimized resource utilization. The primary feedstock, corn starch, undergoes enzymatic hydrolysis facilitated by α-amylase and glucoamylase. These enzymes break down the starch into fermentable sugars such as glucose, which are readily assimilated by fermentative microorganisms.Fermentation...
Bioreactor Controls-III01:22

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
Upstream Processing01:27

Upstream Processing

Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
Methods of Medium Optimization01:28

Methods of Medium Optimization

Optimizing growth media enhances microbial proliferation and maximizes product yield. Statistical experimental design methodologies provide structured and reproducible approaches, offering progressively higher levels of robustness and efficiency.The One-Factor-at-a-Time (OFAT) MethodThe One-Factor-at-a-Time (OFAT) method involves adjusting a single variable while keeping all others constant. However, it cannot detect interactions between variables, often leading to suboptimal outcomes when...
Batch vs Continuous Culture01:14

Batch vs Continuous Culture

Fermentation is a foundational biotechnological process used to produce pharmaceuticals, biofuels, enzymes, and food additives. Among industrial strategies, batch and continuous fermentation are the two most widely applied. Although both rely on microbial conversion of substrates into desired products, they differ markedly in operation, productivity, and suitability for specific applications.Batch fermentation occurs in a closed system in which nutrient media and inoculum are added at the...
Bioreactor Design and Operational System01:29

Bioreactor Design and Operational System

Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

External validation of established clinical risk scores for cancer-associated venous thromboembolism in a Brazilian registry.

Journal of thrombosis and thrombolysis·2026
Same author

Artificial intelligence in computational modeling of thrombosis: Bridging mechanistic insights and clinical translation.

Journal of thrombosis and thrombolysis·2025
Same author

Determination of Patient-Specific Blood Coagulation Kinetic Parameters via Neural Networks: Toward Thrombosis Prediction in Personalized Medicine.

Annals of biomedical engineering·2025
Same author

Integrating biomarkers for hemostatic disorders into computational models of blood clot formation: A systematic review.

Mathematical biosciences and engineering : MBE·2025
Same author

A Comparison of Monoglyceride Production from Microalgaelipids and Rapeseed Oil Catalyzed by Metal Oxides.

ChemSusChem·2024
Same author

Personalized lattice-structured prosthesis as a graftless solution for mandible reconstruction and prosthetic restoration: A finite element analysis.

Journal of the mechanical behavior of biomedical materials·2024
Same journal

Tandem Repeat Gene Strategy for High-Yield Production and Functional Evaluation of Bioactive Wheat Oligopeptides.

Applied biochemistry and biotechnology·2026
Same journal

Blue Carbon Dots Modified Cu-MOF: Excellent Peroxidase-Like Activity and Ratiometric Fluorescence Sensing to L-Cys.

Applied biochemistry and biotechnology·2026
Same journal

Overexpression of human interleukin 6 in NaCl inducible bacterial system and its characterization.

Applied biochemistry and biotechnology·2026
Same journal

From Alga to Consortium: Advancing Petroleum Refinery Wastewater Biotreatment with Picocystis.

Applied biochemistry and biotechnology·2026
Same journal

Bioactive Compound-Integrated Metal Nanoparticles Promote Wound Repair in Diabetic Conditions: Evidence from an Animal Study.

Applied biochemistry and biotechnology·2026
Same journal

DianDao San Promotes CSU Wound Healing by Inhibiting TNF/NF-κB Pathway.

Applied biochemistry and biotechnology·2026
See all related articles

Related Experiment Video

Updated: Jun 27, 2026

Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation
09:28

Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation

Published on: May 18, 2020

Optimization strategies based on sequential quadratic programming applied for a fermentation process for butanol

Adriano Pinto Mariano1, Caliane Bastos Borba Costa, Dejanira de Franceschi de Angelis

  • 1Laboratory of Optimization, Design and Advanced Control (LOPCA), School of Chemical Engineering, University of Campinas (UNICAMP), 3083-970 Campinas, SP, Brazil. adrianomariano@yahoo.com.br

Applied Biochemistry and Biotechnology
|December 17, 2008
PubMed
Summary
This summary is machine-generated.

Mathematical optimization of continuous flash fermentation for biobutanol production was studied. A statistical model proved more robust and faster than a deterministic model for real-time applications.

More Related Videos

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
06:24

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Published on: December 15, 2017

Optimization of Processing of Tiebangchui with Highland Barley Wine Based on the Box-Behnken Design Combined with the Entropy Method
09:12

Optimization of Processing of Tiebangchui with Highland Barley Wine Based on the Box-Behnken Design Combined with the Entropy Method

Published on: May 19, 2023

Related Experiment Videos

Last Updated: Jun 27, 2026

Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation
09:28

Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation

Published on: May 18, 2020

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
06:24

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Published on: December 15, 2017

Optimization of Processing of Tiebangchui with Highland Barley Wine Based on the Box-Behnken Design Combined with the Entropy Method
09:12

Optimization of Processing of Tiebangchui with Highland Barley Wine Based on the Box-Behnken Design Combined with the Entropy Method

Published on: May 19, 2023

Area of Science:

  • Biochemical Engineering
  • Process Optimization
  • Renewable Energy

Background:

  • Continuous fermentation processes offer advantages for biofuel production.
  • Biobutanol is a promising biofuel, but efficient production methods are needed.
  • Flash fermentation coupled with cell retention enhances product recovery.

Purpose of the Study:

  • To mathematically optimize a continuous flash fermentation process for biobutanol production.
  • To maximize biobutanol productivity while achieving desired substrate conversion.
  • To compare the efficacy of deterministic and statistical modeling strategies for process optimization.

Main Methods:

  • The process involved a fermentor, tangential microfiltration for cell retention, and a vacuum flash vessel for continuous butanol recovery.
  • Two optimization strategies were employed: a deterministic model with experimental kinetics and a statistical model using factorial design and simulation.
  • Both strategies formulated the problem as a nonlinear programming problem solved via sequential quadratic programming.

Main Results:

  • Both deterministic and statistical models yielded similar optimization solutions.
  • The deterministic model strategy encountered issues with convergence and required significant computational time.
  • The statistical model strategy demonstrated robustness and speed, making it suitable for real-time applications.

Conclusions:

  • The statistical modeling approach is recommended for optimizing continuous flash fermentation processes due to its speed and robustness.
  • This optimization strategy is well-suited for real-time applications integrating optimization and control.
  • Efficient biobutanol production through optimized flash fermentation contributes to renewable energy goals.