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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Updated: Jun 27, 2026

Microarray Analysis for Saccharomyces cerevisiae

Microarray Analysis for Saccharomyces cerevisiae

Published on: April 7, 2011

Method for conducting microarray study of oxidative stress induced gene expression.

Sita Subbaram¹, Juan A Melendez, Sridar V Chittur

¹Center for Immunology & Microbial Disease, Albany College of Medicine, Albany, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)

|December 17, 2008

Summary

This summary is machine-generated.

This study introduces a method using DNA microarrays to analyze gene expression changes in cells engineered to overexpress antioxidant enzymes. This helps understand cellular responses to oxidative stress.

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Area of Science:

Cellular biology
Molecular biology
Biochemistry

Background:

Cellular metabolism generates reactive oxygen species (ROS).
Oxidative stress impacts gene expression and signaling pathways.
High-throughput methods are needed to study these changes.

Purpose of the Study:

To develop and apply a gene expression microarray method.
To investigate transcriptional changes in redox-engineered cells.
To analyze the effects of mitochondrial antioxidant overexpression.

Main Methods:

Utilized DNA microarrays for gene expression profiling.
Employed redox-engineered cell lines.
Overexpressed manganese superoxide dismutase (MnSOD) and catalase in mitochondria.

Main Results:

Successfully profiled transcriptional changes.
Identified specific gene expression alterations.
Evaluated the impact of MnSOD and catalase overexpression on cellular redox state.

Conclusions:

Gene expression microarrays are effective for studying oxidative stress responses.
Mitochondrial antioxidant overexpression significantly alters cellular transcription.
This method provides insights into cellular redox regulation.