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Updated: Jun 27, 2026

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana
Published on: May 21, 2019
Phenotyping of abiotic responses and hormone treatments in Arabidopsis
1Ludwig-Maximilians-Universität, Department für Biologie I, Lehrstuhl für Botanik, München, Germany.
Abstract:
The disruption or modulation of signal transduction pathways does not always lead to drastic changes in plant growth and development. Therefore, many loss- or gain-of-function lines do not exhibit an obvious phenotype under normal greenhouse conditions. To be able to assign biological functions to these genes, the mutants need to be evaluated with a broad spectrum of assays to uncover conditional phenotypes.Here we provide an overview on how to evaluate plants in their development and their response to abiotic factors such as light, hormones, and different stressors. The assessment of the behavior of a plant under these conditions can be used to correlate a biological role with a genotype. This phenotypic analysis can be used for profiling of mutants.
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Transcription
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Plant Hormones
Cell Signaling in Plants
Responses to Heat and Cold Stress