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Related Experiment Video

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Live Calcium Imaging of Virus-Infected Human Intestinal Organoid Monolayers Using Genetically Encoded Calcium Indicators

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Rotavirus VP7-gene selection during coinfections in CaCo-2 cells.

P A Barril¹, M O Giordano, G Masachessi

¹Instituto de Virología "Dr. J. M. Vanella", Facultad de Ciencias Médicas, Universidad Nacional de Córdoba. Enfermera Gordillo Gómez s/n., Ciudad Universitaria, (5000) Córdoba, Argentina.

Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases

|December 17, 2008

Summary

Rotavirus G-type replacement dynamics were studied using competitive growth in cell cultures. G1 strains showed preferential selection, while G1+G4 coinfections were co-amplified, offering insights into rotavirus circulation patterns.

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Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus

Published on: January 28, 2019

Area of Science:

Virology
Epidemiology
Molecular Biology

Background:

Rotavirus epidemiological surveys in Córdoba, Argentina, show a high frequency of mixed G-type infections (41.5%).
The most prevalent mixed G-type combinations observed were G1+G4 (65.0%), G1+G2 (12.5%), G2+G4 (3.1%), and G1+G9 (2.5%).

Purpose of the Study:

To analyze the competitive growth of rotavirus strains from frequent coinfections in cell cultures.
To understand the dynamic of G-type replacement over time by examining differential strain fitness.

Main Methods:

Utilized CaCo-2 cell cultures to assess the competitive growth of rotavirus strains.
Analyzed strains from the most frequent rotavirus G-type coinfections identified in previous epidemiological surveys.

Main Results:

Rotavirus G1-type demonstrated preferential selection over G2 and G9 genotypes.
G1+G4 coinfections exhibited efficient co-amplification, mirroring high detection rates in the region.
Rotavirus G2-type showed a better amplification rate compared to G4-type.

Conclusions:

Competitive replication in vitro provides a valid method for understanding rotavirus G-type circulation patterns.
Differential growth characteristics of rotavirus strains in culture may explain fluctuating genotype prevalence over time.
Findings support the hypothesis that strain fitness, measurable by differential growth, influences rotavirus G-type dynamics.