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Published on: June 9, 2021
A new method for determination of myricetin and quercetin using solid phase microextraction-high performance liquid
Ashwini Kumar1, Ashok Kumar Malik, Dhananjay Kumar Tewary
1Department of Chemistry, Punjabi University, Patiala 147 002, India.
Abstract:
A new approach for the extraction and determination of myricetin and quercetin by using SPME-HPLC-UV system has been developed. The method involves adsorption of flavonoids on CAR/TPR fiber followed by desorption in the desorption chamber of SPME-HPLC interface using citrate buffer (0.001 M):acetonitrile (70:30) as mobile phase and UV detection at 372 nm. The detection limits for myricetin and quercetin are 48.3 and 24.7 pgmL(-1), respectively. The proposed method was validated by determining myricetin and quercetin in tomato, onion, grapes and red wine samples.
