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Related Concept Videos

Cell Signaling in Plants01:25

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
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The Equilibrium Binding Constant and Binding Strength

The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:

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Related Experiment Video

Updated: Jun 27, 2026

BRET-based G Protein Biosensors for Measuring G Protein-Coupled Receptor Activity in Live Cells
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Binding assays for brassinosteroid receptors.

Ana Caño-Delgado1, Zhi-Yong Wang

  • 1Departamento de Genética Molecular, Consorcio CSIC-IRTA, Barcelona, Spain.

Methods in Molecular Biology (Clifton, N.J.)
|December 17, 2008
PubMed
Summary

Brassinosteroid (BR) receptors, like BRI1 in Arabidopsis, directly bind plant hormones. This binding occurs within a specific subdomain of the BRI1 extracellular domain, crucial for brassinosteroid signaling.

Area of Science:

  • Plant molecular biology
  • Receptor kinase signaling
  • Plant hormone research

Background:

  • BRI1 is a leucine-rich repeat receptor kinase in Arabidopsis thaliana.
  • BRI1 is a key component of the brassinosteroid (BR) receptor complex.
  • Previous studies identified BRI1 and BRI1-like proteins (BRL1, BRL3) as in vivo BR receptor complex components.

Purpose of the Study:

  • To investigate the direct binding of brassinosteroids to the BRI1 receptor.
  • To identify the specific region within BRI1 responsible for brassinosteroid perception.
  • To further elucidate the mechanism of brassinosteroid signaling in Arabidopsis.

Main Methods:

  • BR-binding assays in Arabidopsis plants.
  • Utilizing previously established experimental procedures.

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  • Employing photo-affinity labeled castasterone for binding studies.

    • Main Results:

    • Brassinosteroids (BRs) are directly perceived by the BRI1 receptor.
    • Binding occurs within a specific subdomain of BRI1's extracellular domain.
    • This critical subdomain is defined by 94 amino acids within ID-LRR22.

    Conclusions:

    • The extracellular domain of BRI1 directly interacts with brassinosteroids.
    • A specific 94-amino acid region (ID-LRR22) is essential for BR perception by BRI1.
    • This finding refines our understanding of the brassinosteroid receptor complex and hormone signaling.