Related Experiment Video
Updated: Jun 27, 2026
06:14
Isolation of Mouse Pancreatic Endothelial Cells
Published on: June 21, 2024
Enzyme kinetics and characterization of mouse pancreatic elastase
Deepa Nadarajah1, Mark A L Atkinson, Paul Huebner
1Department of Biochemistry, University of Texas Health Center at Tyler, Tyler, Texas 75708, USA.
Connective Tissue Research
|December 17, 2008
Summary
Researchers purified and characterized mouse pancreatic elastase, a serine protease. The enzyme
Area of Science:
- Biochemistry
- Enzymology
- Proteomics
Background:
- Pancreatic elastases are key digestive enzymes.
- Understanding murine pancreatic elastase is crucial for comparative enzymology.
Purpose of the Study:
- To purify and characterize mouse pancreatic elastase.
- To compare its properties with other known elastases.
Main Methods:
- Enzyme extraction from mouse pancreas.
- Ammonium sulfate precipitation and ion exchange chromatography.
- SDS-PAGE, ultracentrifugation, and kinetic analysis.
Main Results:
- Purified mouse pancreatic elastase to a single band.
- Determined molecular weight (25.9 kDa) and frictional ratio (1.26).
- Identified pH optimum of 8.0 and characterized kinetic properties.
Conclusions:
- Mouse pancreatic elastase exists as an activatable proform.
- Enzymatic properties are similar to other serine elastases.
- Provides a basis for further functional studies.
Related Concept Videos
01:19Enzyme Kinetics
Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
01:19Introduction to Enzyme Kinetics
Enzyme kinetics studies the rates of biochemical reactions. Scientists monitor the reaction rates for a particular enzymatic reaction at various substrate concentrations. Additional trials with inhibitors or other molecules that affect the reaction rate may also be performed.
The experimenter can then plot the initial reaction rate or velocity (Vo) of a given trial against the substrate concentration ([S]) to obtain a graph of the reaction properties. For many enzymatic reactions involving a...
The experimenter can then plot the initial reaction rate or velocity (Vo) of a given trial against the substrate concentration ([S]) to obtain a graph of the reaction properties. For many enzymatic reactions involving a...
01:30Enzyme Inhibition
Inhibitors are molecules that reduce enzyme activity by binding to the enzyme. In a normally functioning cell, enzymes are regulated by a variety of inhibitors. Drugs and other toxins can also inhibit enzymes. Some inhibitors bind to the enzyme’s active site, while others inhibit enzymatic activity by binding to other sites on the protein structure.