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Updated: Jun 27, 2026

Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches
Published on: October 27, 2013
Relationships between free living amoebae and Exophiala dermatitidis: a preliminary study
Estelle Cateau1, Tiphaine Mergey, Catherine Kauffmann-Lacroix
1Laboratoire de parasitologie et mycologie médicale, CHU La Milétrie, Poitiers, France. estelle.cateau@chu-poitiers.fr
Abstract:
Free living amoebae can play a role as reservoirs for pathogens isolated from hospital water. We have investigated the potential interactions between two protozoa (Acanthamoeba castellanii and Hartmanella vermiformis) that may be recovered from hospital water tips and Exophiala dermatitidis, a black yeast often recovered from water sources. We showed that the presence of trophozoites or supernatants of culture of H. vermiformis increased fungal growth, whereas the same phenomenon was observed only with the supernatant of A. castellanii cultures. These preliminary results highlight the fact that the recovering of free-living amoebae in hospital water taps could lead to the development of fungal nosocomial pathogens.
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