Improved neutrophil separation from anti-D-treated Rh(D)-positive whole blood by a discontinuous gradient method

Neutrophil separation in Rh(D)-positive whole blood with a discontinuous gradient method (Ficoll 1077-1119 and sodium diatrizoate) was improved by adding anti-D reagent to ethylene diaminetetraacetic acid anticoagulated blood (1 drop/mL). The mean yield was raised more than two times (x2.6) without red blood cell contamination. Anti-D reagent did not impair neutrophil viability, purity, nitroblue tetrazolium-formazan production, or shape change response to N-formyl-methionyl-leucinyl-phenyl-alanine.