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RBM19 is essential for preimplantation development in the mouse.

Jian Zhang¹, Amber J Tomasini, Alan N Mayer

¹Division of Gastroenterology, Department of Pediatrics, Medical College of Wisconsin and Children's Research Institute, Milwaukee, WI 53226, USA. jzhang@mcw.edu

BMC Developmental Biology

|December 18, 2008

Summary

RNA-binding motif protein 19 (RBM19) is crucial for early mouse development. Loss of RBM19 function arrests embryonic development at the morula stage due to impaired nucleologenesis.

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Area of Science:

Developmental Biology
Molecular Biology
Genetics

Background:

RNA-binding motif protein 19 (RBM19) is a conserved nucleolar protein involved in rRNA processing for ribosome biogenesis.
RBM19 has a known role in digestive organ development in zebrafish.

Purpose of the Study:

To investigate the function of RBM19 during mouse embryonic development.
To analyze the consequences of RBM19 deficiency in early embryogenesis.

Main Methods:

Generation of Rbm19 mutant mice using gene-trap insertion.
Analysis of embryonic development, nucleologenesis, apoptosis, and gene expression in mutant embryos.

Main Results:

Homozygous Rbm19 mutant embryos arrested development at the morula stage.

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Mutant embryos exhibited defective nucleologenesis, increased apoptosis, and P53 target gene upregulation.

RBM19 mislocalization to the cytoplasm was observed in morula-stage mutant embryos.

Conclusions:

RBM19 is essential for preimplantation development in mice.
De novo nucleologenesis is critical for successful early embryonic development.