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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Cleavage and Blastulation01:33

Cleavage and Blastulation

After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
Gastrulation01:56

Gastrulation

Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata will form...
Development of the Sexual Organs in the Embryo and Fetus01:15

Development of the Sexual Organs in the Embryo and Fetus

Development of the reproductive organs in an embryo starts from a bipotential state. This means the early embryo can develop either male or female reproductive organs. The formation of these organs begins with the growth of gonadal ridges that arise from the intermediate mesoderm during the fifth week of development.
Near the gonadal ridges, two duct systems are present: the mesonephric ducts (Wolffian ducts) and paramesonephric ducts (Müllerian ducts). These ducts form the basis for the male...
Embryonic Stem Cells00:58

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
Embryonic Stem Cells00:57

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

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Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing (Frog) Embryo
09:18

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing (Frog) Embryo

Published on: April 21, 2022

The human embryo proteome.

F Dominguez1, A Pellicer, C Simón

  • 1Fundación IVI, Instituto Universitario IVI, Universidad de Valencia, Valencia, Spain. fdominguez@ivi.es

Reproductive Sciences (Thousand Oaks, Calif.)
|December 18, 2008
PubMed
Summary
This summary is machine-generated.

Understanding the human embryo proteome is crucial for improving assisted reproductive technologies. This study reviews methods to analyze the blastocyst secretome and implantome for noninvasive embryo selection.

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Published on: January 30, 2017

Area of Science:

  • Reproductive biology
  • Proteomics
  • Embryology

Background:

  • Limited knowledge exists on the human preimplantation embryo's proteome, particularly the blastocyst's protein composition before implantation.
  • Current methods for assessing embryo viability in assisted reproductive technologies primarily rely on morphological evaluation.
  • Legal and ethical considerations necessitate novel approaches for studying the human embryo proteome.

Purpose of the Study:

  • To investigate the protein secretion and consumption profile of the human blastocyst (secretome) and implanted blastocyst (implantome).
  • To develop novel noninvasive models for blastocyst selection.
  • To review existing information on the human proteome using various techniques.

Main Methods:

  • Review of scientific literature on human embryo proteome analysis.
  • Exploration of techniques for secretome and implantome profiling.
  • Discussion of noninvasive methods for assessing proteomic and metabolic status.

Main Results:

  • The study highlights the need for advanced techniques beyond morphological assessment.
  • It emphasizes the potential of secretome and implantome analysis for embryo selection.
  • The review consolidates information on different approaches to human proteome studies.

Conclusions:

  • Noninvasive methods, particularly analyzing the blastocyst secretome and implantome, offer promising alternatives for embryo selection in assisted reproduction.
  • Further research into embryo proteomic and metabolic status is essential.
  • Developing these noninvasive models can enhance the success rates of assisted reproductive technologies.