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Updated: Jun 27, 2026
Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
Published on: April 12, 2015
[Study on human neural stem cells differentiation in vitro and transplantation in nude mice]
Zhi Qiang Li1, Li Ping Duan, Li Shen
1Department of Gastroenterology, Peking University Third Hospital, Beijing, China.
Objective:
To investigate the differentiation of human neural stem cells (hNSCs) in vitro and to assess the potential of hNSCs transplanted into the pylorus of nude mice.
Methods:
Central nervous system-derived hNSCs (CNS-hNSCs) were obtained from the subventricular zone of aborted fetus brain (10 weeks), suspension cultured, passaged and induced to differentiate in vitro. Stem cell marker neuroepithelial stem cell protein (nestin), enteric neuronal marker protein gene production 9.5 (PGP9.5) and glial cell marker glial fibrillary acidic protein (GFAP) were detected by immunofluorescence method. CNS-hNSCs (cultured for 3 months) were transplanted into the pylorus of nude mice and followed by immunofluorescence method.
Results:
Using the immunofluorescence method, nestin could be identified and the induced cells expressed PGP9.5 and GFAP. After being transplanted into the pylorus of nude mice, CNS-hNSC could be detected to survive at least for 6 weeks.
Conclusion:
CNS-hNSCs could be cultured, passaged and induced in vitro successfully; transplantation of CNS-hNSCs into gastrointestinal tract could be a potential cellular replacement strategy for gastrointestinal motility disorders.
