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Published on: April 18, 2019
In vitro efficacy for some local antimicrobial products against E. coli K12
Azizollah Ebrahimi1, Abdolkarim Zamani Moghadam, Mostafa Shakhse Niaie
1Department of Pathobiology, School of Veterinary Science, Shahrekord University, P.O. Box 115, Shahrekord, Iran.
Abstract:
The aim of this study was to assess the in vitro activity of a number of antimicrobial agents (Sultrim, Oxytetracycline, enrofloxacin and florfenicol) produced by different Iranian manufacturers against E. coli K12. Comparison of the MICs of reference antimicrobials with Disk Diffusion (DD) results of different products made by Iranian manufacturers revealed that all products of tested antimicrobials seem to be in synergy with their reference MIC evaluation. Present data indicate that E. coli K 12 is resistant to Enrofloxacin (MIC value of 6.25 microg mL(-1)) by both MIC and DD evaluation.
Insights
This study evaluated antimicrobial effectiveness against E. coli K12. Results show E. coli K12 is resistant to enrofloxacin, with other tested antimicrobials showing synergy with reference evaluations.
Area of Science:
- Microbiology and Infectious Diseases
- Pharmacology and Pharmaceutical Sciences
Background:
- Escherichia coli (E. coli) K12 is a common laboratory strain used in microbiological research.
- Antimicrobial resistance is a growing global health concern, necessitating continuous evaluation of drug efficacy.
Purpose of the Study:
- To determine the in vitro activity of various antimicrobial agents against E. coli K12.
- To compare the efficacy of antimicrobial products from different Iranian manufacturers.
Main Methods:
- Minimum Inhibitory Concentration (MIC) assays were performed for selected antimicrobials.
- Disk Diffusion (DD) testing was utilized to assess antimicrobial susceptibility.
- Reference antimicrobials were compared against products from Iranian manufacturers.
Main Results:
- All tested antimicrobial products showed synergy with their reference MIC evaluations.
- E. coli K12 demonstrated resistance to enrofloxacin, with a notable MIC value of 6.25 µg/mL.
- Resistance to enrofloxacin was confirmed by both MIC and Disk Diffusion methods.
Conclusions:
- The study highlights the in vitro efficacy of several antimicrobial agents against E. coli K12.
- A significant finding is the observed resistance of E. coli K12 to enrofloxacin.
- Iranian-manufactured antimicrobial products appear consistent with reference evaluations.
