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Updated: Jan 30, 2026

Identification of Hemolytic and Phospholipase Activity in Crude Extracts from Sea Anemones by Straightforward Bioassays
Published on: March 29, 2022
Hemolytic activity of Trichomonas gallinae isolates does not correspond with clinical virulence
Richard W Gerhold1, Michael J Yabsley, John R Fischer
1College of Veterinary Medicine, The University of Georgia, Athens, GA 30602, USA. rgerhold@uga.edu
Abstract:
The hemolytic activity of 22 Trichomonas gallinae isolates was investigated using an 18h erythrocyte hemolysis assay which has been shown to correlate with the clinical virulence of T. vaginalis. Absorbance of the assay supernatants was measured at 540nm and expressed as percentage of complete hemolysis. Mean hemolytic activity of the T. gallinae isolates ranged from 3.5% to 53.4% and did not correspond with clinical virulence. The results of this investigation suggest hemolytic activity is not a useful in vitro virulence assay for T. gallinae.
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