Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tuning the Reactivity of Al@TiO<sub>2</sub> Antenna-Reactor Plasmonic Photocatalysts by Controlling Oxygen Vacancies.

Nano letters·2025
Same author

Tailoring the aluminum nanocrystal surface oxide for all-aluminum-based antenna-reactor plasmonic photocatalysts.

Proceedings of the National Academy of Sciences of the United States of America·2024
Same author

Electrocatalytic activity and surface oxide reconstruction of bimetallic iron-cobalt nanocarbide electrocatalysts for the oxygen evolution reaction.

RSC advances·2023
Same author

Cool carriers: triplet diffusion dominates upconversion yield.

Nanoscale·2023
Same author

Effective Mass for Holes in Paramagnetic, Plasmonic Cu<sub>5</sub>FeS<sub>4</sub> Semiconductor Nanocrystals.

The journal of physical chemistry. C, Nanomaterials and interfaces·2023
Same author

Influence of Al<sub>2</sub>O<sub>3</sub> Overlayers on Intermolecular Interactions between Metal Oxide Bound Molecules.

Molecules (Basel, Switzerland)·2023

Related Experiment Video

Updated: Jun 27, 2026

Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy
08:17

Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy

Published on: August 16, 2021

Tracking spatial disorder in an optical ruler by time-resolved NSET.

Mani Prabha Singh1, Travis L Jennings, Geoffrey F Strouse

  • 1Department of Chemistry and Biochemistry, Molecular Biophysics Program, Florida State University, Tallahassee, Florida 32306-4390, USA.

The Journal of Physical Chemistry. B
|December 23, 2008
PubMed
Summary

This study introduces nanometal surface energy transfer (NSET) to assess dye-biomolecule interactions, crucial for optical ruler accuracy. NSET reveals specific DNA interactions for Cy5 dye, unlike non-interacting FAM.

More Related Videos

Measuring Attention and Visual Processing Speed by Model-based Analysis of Temporal-order Judgments
13:00

Measuring Attention and Visual Processing Speed by Model-based Analysis of Temporal-order Judgments

Published on: January 23, 2017

Sample Drift Correction Following 4D Confocal Time-lapse Imaging
10:04

Sample Drift Correction Following 4D Confocal Time-lapse Imaging

Published on: April 12, 2014

Related Experiment Videos

Last Updated: Jun 27, 2026

Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy
08:17

Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy

Published on: August 16, 2021

Measuring Attention and Visual Processing Speed by Model-based Analysis of Temporal-order Judgments
13:00

Measuring Attention and Visual Processing Speed by Model-based Analysis of Temporal-order Judgments

Published on: January 23, 2017

Sample Drift Correction Following 4D Confocal Time-lapse Imaging
10:04

Sample Drift Correction Following 4D Confocal Time-lapse Imaging

Published on: April 12, 2014

Area of Science:

  • Biophysics
  • Molecular Biology
  • Nanotechnology

Background:

  • Dye-biomolecule interactions impact optical ruler accuracy.
  • Nanometal surface energy transfer (NSET) offers a novel optical technique.
  • Direct assessment of dye-DNA interactions is needed.

Purpose of the Study:

  • Develop and demonstrate NSET for assessing dye-DNA interactions.
  • Quantify interactions of carboxyfluorescein (FAM) and Cy5 dyes with DNA.
  • Provide insights for optimizing dye usage in FRET-based optical ruler technologies.

Main Methods:

  • Utilized nanometal surface energy transfer (NSET) as an optical technique.
  • Probed interactions between DNA and carboxyfluorescein (FAM) and Cy5 dyes.
  • Analyzed dye interactions based on appendage site and linker arm length.

Main Results:

  • FAM showed no interaction with the DNA backbone, behaving as a solvent-exposed dye.
  • Cy5 dye exhibited two distinct interactions with DNA, dependent on labeling site and linker length.
  • Quantified Cy5 intercalation ratios: 37% for internal C(6) and 42% for internal C(3) spacers; no interaction for terminal labeling.

Conclusions:

  • NSET enables direct assessment of dye-DNA interactions without single-molecule methods.
  • Quantitative data on dye site occupation is achievable, improving FRET-based optical ruler applications.
  • Understanding dye-biomolecule interactions is critical for accurate molecular measurements.