Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Principles Of Column Chromatography

Principles Of Column Chromatography

The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...

Chromatography: Introduction

Chromatography: Introduction

Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...

Chromatographic Methods: Classification

Chromatographic Methods: Classification

Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface.
Chromatographic techniques are typically named by...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Standardized HPTLC for Reproducible Chemical Fingerprinting.

Methods in molecular biology (Clifton, N.J.)·2025

Same author

A U.S. Pharmacopeia (USP) overview of Pan American botanicals used in dietary supplements and herbal medicines.

Frontiers in pharmacology·2024

Same author

Quality variation of maidong (Ophiopogon japonicus and Liriope spicata) - A HPTLC-based approach.

Journal of pharmaceutical and biomedical analysis·2024

Same author

Comprehensive high-performance thin-layer chromatography analysis of <i>Monteverdia ilicifolia</i> leaf and its adulterants.

Natural product research·2023

Same author

Investigation of market herbal products regulated under different categories: How can HPTLC help to detect quality problems?

Frontiers in pharmacology·2022

Same author

Applicability of the Universal Mixture for describing system suitability and quality of analytical data in routine normal phase High Performance Thin Layer Chromatography methods.

Journal of chromatography. A·2022

Same journal

Phytochemicals to Pharmacology: Potential Cytotoxic and Tumor Inhibitory Activity of Elaeocarpus ganitrus Extract in Preclinical Models.

Planta medica·2026

Same journal

Edible Plants with Antimicrobial Potential - Report of the Expert Meetings from Ibero-American Countries.

Planta medica·2026

Same journal

Ginsenoside Rd ameliorates glucose metabolism by regulating GLP-1/ MAPK/ NF-κB signaling pathway in db/db mice.

Planta medica·2026

Same journal

Therapeutic Potential of Limonium brasiliense Extracts in Helicobacter pylori-Associated Gastric Pathologies.

Planta medica·2026

Same journal

Phytochemical Study and Antibacterial Activity of Cedrus atlantica Aerial Part Apolar Extract.

Planta medica·2026

Same journal

Selection of Promising Candidates for Herbal Veterinary Medicinal Products: A Strategic Approach.

Planta medica·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 26, 2026

A Simple Protocol for Mapping the Plant Root System Architecture Traits

A Simple Protocol for Mapping the Plant Root System Architecture Traits

Published on: February 10, 2023

Plant analysis 2008--planar chromatography.

Eike Reich¹, Valeria Widmer

¹CAMAG Laboratory, Muttenz, Switzerland. eike.reich@camag.com

|December 23, 2008

Summary

This summary is machine-generated.

High-performance thin-layer chromatography (HPTLC) remains vital for plant analysis, aiding in identifying medicinal plants and supplements. Advancements ensure reliable results for quality control and authentication.

More Related Videos

Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue

Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue

Published on: October 6, 2022

Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds

Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds

Published on: March 27, 2014

Related Experiment Videos

Last Updated: Jun 26, 2026

A Simple Protocol for Mapping the Plant Root System Architecture Traits

A Simple Protocol for Mapping the Plant Root System Architecture Traits

Published on: February 10, 2023

Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue

Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue

Published on: October 6, 2022

Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds

Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds

Published on: March 27, 2014

Area of Science:

Analytical Chemistry
Pharmacognosy
Plant Sciences

Background:

Planar chromatography has a 70-year history in plant analysis.
High-performance thin-layer chromatography (HPTLC) is a modern, advanced form of this technique.
HPTLC is crucial for identifying medicinal plants and dietary supplements.

Purpose of the Study:

To highlight the enduring importance and advancements in HPTLC for plant analysis.
To showcase HPTLC's role in detecting adulteration and quantifying marker substances.
To discuss the integration of new technologies enhancing HPTLC's capabilities.

Main Methods:

Utilizing advanced instrumentation and methodological concepts for reliable HPTLC results.
Implementing improved control over chromatogram development.
Employing image analysis for sample information acquisition and comparison.

Main Results:

Significant progress in managing the planar chromatographic process.
Enhanced reliability in both qualitative and quantitative HPTLC analyses.
Expanded applicability through biological detection and mass spectrometry hyphenation.

Conclusions:

HPTLC is a robust technique for the identification and quality control of plant-derived products.
Technological advancements have improved the precision and scope of HPTLC.
HPTLC serves as a valuable orthogonal and complementary technique to High-Performance Liquid Chromatography (HPLC).