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Related Concept Videos

High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

1.4K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
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High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

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High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
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Related Experiment Video

Updated: May 30, 2025

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
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Standardized HPTLC for Reproducible Chemical Fingerprinting.

Thi Kieu Tiên Do1,2, Ilona Trettin3,4, Eike Reich4

  • 1CAMAG, Muttenz, Switzerland. tien.do@camag.com.

Methods in Molecular Biology (Clifton, N.J.)
|January 30, 2025
PubMed
Summary
This summary is machine-generated.

This study presents a standard operating procedure for high-performance thin-layer chromatography (HPTLC) to analyze natural products. The method ensures reproducible chemical fingerprinting for natural product databases.

Keywords:
Complementary developing solvent (CDS)FingerprintingStandard operating procedureStandardizationUniversal HPTLC mixture (UHM)

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Area of Science:

  • Analytical Chemistry
  • Natural Product Chemistry

Background:

  • High-performance thin-layer chromatography (HPTLC) is vital for natural product (NP) chemical fingerprinting.
  • Standardized methods are essential for reproducible NP analysis.

Purpose of the Study:

  • To establish a comprehensive standard operating procedure (SOP) for HPTLC analysis of natural products.
  • To enable reproducible chemical fingerprinting of diverse natural product samples.

Main Methods:

  • Development of a non-targeted screening approach for HPTLC.
  • Implementation of a two-step derivatization process for broad phytochemical detection.
  • Adaptation of the SOP for both manual and automated HPTLC instruments.

Main Results:

  • A detailed SOP for HPTLC analysis of natural products was established.
  • The method ensures reproducible results for chemical fingerprinting.
  • The procedure is applicable to a wide range of phytochemical classes.

Conclusions:

  • The presented HPTLC SOP provides a reliable method for natural product analysis.
  • Consistent results can be achieved, facilitating the creation of NP databases.
  • This standardized approach enhances the utility of HPTLC in natural product research.