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Updated: Aug 13, 2026

Alternative Methods for the Detection of Superoxide Anion Generation in Platelets
Published on: March 29, 2024
Superoxide dismutase triggers activation of "primed" platelets
L Iuliano1, D Praticò, A Ghiselli
1Institute of 1st Clinical Medicine, University of Rome, La Sapienza, Italy.
Abstract:
Superoxide dismutase (SOD) triggers activation of human platelets exposed to subthreshold concentrations of arachidonic acid and collagen. The subthreshold concentrations used are not able to activate platelets but "prime" platelets to be activated by SOD. The addition of SOD to arachidonic acid-or collagen-primed platelets induced aggregation, thromboxane A2 production, and release of [3H]serotonin. Superoxide dismutase does not have any effect on resting platelets and ADP-, thrombin-, calcium ionophore A23187-, PAF-, or U46619-stimulated platelets. Furthermore, superoxide dismutase-dependent platelet activation is fully prevented by catalase and/or aspirin, suggesting a role for H2O2 and the involvement of the cyclooxygenase pathway of arachidonic acid in such activation.
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