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Monoclonal anti-A and anti-A,B antibodies from a mouse immunized with A secretor saliva
S Miyazaki1, T Nakajima, K Furukawa
1Department of Legal Medicine, Gunma University School of Medicine, Maebashi, Japan.
Using spleen cells from a mouse immunized with blood group A secretor saliva, we obtained 3 monoclonal anti-A antibodies (3H9, 5C6, 4D8) and 1 monoclonal anti-A,B antibody (2D10). In agglutination tests against red cells of A subgroups, 3H9 gave similar results to human anti-A serum, and 2D10 reacted with both A and B cells. 5C6 agglutinated especially A1 cells, while 4D8 showed stronger agglutination of A2 cells than the other antibodies. Using these antibodies, it is possible to distinguish the subgroups of A. The 4 antibodies had no reactivity with the red cells of 6 animal species and nonhuman primates except for group A anthropoid apes. All 4 antibodies were negative with Cad-positive, sheep and papain-treated O red cells that are agglutinated by the A-cell-reactive lectins. The activity of 3H9 was inhibited by A secretor saliva and that of 2D10 by both A and B secretor salivas. 4D8 showed a weak reaction with A secretor saliva. Antibody 5C6 was inhibited rather strongly even by A nonsecretor saliva. Thus, 5C6 is useful for application to test specimens in legal medicine, such as examination of blood or salivary stains for A antigens.
Using spleen cells from a mouse immunized with blood group A secretor saliva, we obtained 3 monoclonal anti-A antibodies (3H9, 5C6, 4D8) and 1 monoclonal anti-A,B antibody (2D10). In agglutination tests against red cells of A subgroups, 3H9 gave similar results to human anti-A serum, and 2D10 reacted with both A and B cells. 5C6 agglutinated especially A1 cells, while 4D8 showed stronger agglutination of A2 cells than the other antibodies. Using these antibodies, it is possible to distinguish the subgroups of A. The 4 antibodies had no reactivity with the red cells of 6 animal species and nonhuman primates except for group A anthropoid apes. All 4 antibodies were negative with Cad-positive, sheep and papain-treated O red cells that are agglutinated by the A-cell-reactive lectins. The activity of 3H9 was inhibited by A secretor saliva and that of 2D10 by both A and B secretor salivas. 4D8 showed a weak reaction with A secretor saliva. Antibody 5C6 was inhibited rather strongly even by A nonsecretor saliva. Thus, 5C6 is useful for application to test specimens in legal medicine, such as examination of blood or salivary stains for A antigens.