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Updated: Jun 26, 2026

A Coregistered Ultrasound and Photoacoustic Imaging Protocol for the Transvaginal Imaging of Ovarian Lesions
Published on: March 3, 2023
True ovarian volume is underestimated by two-dimensional transvaginal ultrasound measurement
Mikkel Rosendahl1, Erik Ernst, Per Emil Rasmussen
1Laboratory of Reproductive Biology, Copenhagen University Hospital, Rigshospitalet, Blegdamsvej 9, 2100 Copenhagen, Denmark. mikkel.rosendahl@rh.regionh.dk
Objective:
To investigate a possible difference between true ovarian volume and ovarian volume estimated with two-dimensional (2D) transvaginal ultrasound.
Design:
Prospective clinical and laboratory study.
Setting:
University hospital research laboratory.
Patient(S):
Premenopausal girls and women from three Danish national fertility centers (A: n = 42; B: n = 6; C: n = 18), who had one entire ovary removed for cryopreservation of ovarian cortex.
Intervention(S):
Transvaginal 2D ultrasound measurement of ovarian volume before oophorectomy. True ovarian volume was obtained by weighing the ovary.
Main Outcome Measure(S):
Ovarian volume estimated by weight and ultrasound.
Result(S):
Ovarian tissue density was 1.00 g/mL. Mean ovarian volume by ultrasound vs. weight in the three groups was as follows: A: 6.3 mL vs. 7.8 mL; B: 5.4 mL vs. 6.8 mL; and C: 2.8 mL vs. 6.1 ml. Ovarian volume obtained by ultrasound was at least 27% smaller than the true ovarian volume.
Conclusion(S):
Ovarian volume was severely underestimated by transvaginal 2D ultrasound measurement.
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