Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a rapamycin-insensitive companion...
Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Resident mesenchymal progenitor cells require autocrine IGF-I in homeostatic and regenerating skeletal muscle.

Stem cell reports·2026
Same author

Enhanced Pro-Osteogenic Regulatory Modulation in Mesenchymal Stem Cells Derived from the Periosteum Under Simulated Microgravity.

Cells·2026
Same author

Vps35 p. D620N causes Lrrk2 kinase hyperactivity, chronic microglial activation and inflammation.

bioRxiv : the preprint server for biology·2026
Same author

Knockdown of RNA editing proteins reshapes the HepaRG transcriptome and pharmacogene expression.

Scientific reports·2026
Same author

Atorvastatin Protects Against Deleterious Carfilzomib-Induced Transcriptional Changes in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

International journal of molecular sciences·2026
Same author

Alterations in gene expression and DNA methylation in the bovine blastocyst in response to choline supplementation during culture†.

Biology of reproduction·2026
Same journal

TaSPL14-D diverged from its ortholog to regulate tiller angle in rice: a caveat for orthology-based functional inference.

Plant physiology·2026
Same journal

From wrinkled seeds to plant oil accumulation networks: The legacy of a Plant Physiology classic.

Plant physiology·2026
Same journal

LcHXK1 mediates glucose signaling to inhibit fruit abscission by phosphorylating LcWRKY42, a feedback regulator in lignin polymerization.

Plant physiology·2026
Same journal

Partial submergence-induced adventitious root emergence in cucumber requires CsRBOHB-mediated ROS production.

Plant physiology·2026
Same journal

JA differentially regulates a SmWLIM1/MYB62-SUS1 module to control male fertility via starch biosynthesis.

Plant physiology·2026
Same journal

The chloroplastic NFU1 maturation factor sustains iron-sulfur cluster assembly in the dark in Chlamydomonas.

Plant physiology·2026
See all related articles

Related Experiment Video

Updated: Jun 26, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

A rice kinase-protein interaction map.

Xiaodong Ding1, Todd Richter, Mei Chen

  • 1Department of Plant Pathology , University of Florida, Gainesville, Florida 32611, USA.

Plant Physiology
|December 26, 2008
PubMed
Summary
This summary is machine-generated.

This study maps rice protein kinase interactions using yeast two-hybrid, revealing new insights into plant defense signaling. The findings provide a foundation for future research on kinase functions in rice and other plants.

More Related Videos

Identification of Kinase-substrate Pairs Using High Throughput Screening
11:13

Identification of Kinase-substrate Pairs Using High Throughput Screening

Published on: August 29, 2015

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein
11:23

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

Published on: June 30, 2019

Related Experiment Videos

Last Updated: Jun 26, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Identification of Kinase-substrate Pairs Using High Throughput Screening
11:13

Identification of Kinase-substrate Pairs Using High Throughput Screening

Published on: August 29, 2015

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein
11:23

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

Published on: June 30, 2019

Area of Science:

  • Plant Biology
  • Molecular Biology
  • Genomics

Background:

  • Plants possess numerous protein kinases with largely unknown functions.
  • Genetic studies often lack observable phenotypes, necessitating new methods to understand kinase roles.

Purpose of the Study:

  • To generate a protein interaction map for rice kinases.
  • To identify novel functional insights and potential roles in plant signaling pathways.

Main Methods:

  • Utilized a high-throughput yeast two-hybrid system for large-scale protein interaction mapping.
  • Analyzed interactions of 116 rice kinases with 254 interacting proteins.

Main Results:

  • Developed a comprehensive protein interaction map for rice kinases.
  • Identified a potential role for E3 ubiquitin ligases in plant pathogen defense signaling via receptor-like kinases.
  • Highlighted kinases from expanded subfamilies in rice potentially involved in defense.

Conclusions:

  • The generated interaction map supports known and predicts novel kinase-protein interactions.
  • Provides a foundation for targeted functional studies in rice and other plant species.
  • Demonstrates the utility of yeast two-hybrid for large-scale plant protein interaction studies.