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Monitoring patterns of gene expression in Dictyostelium by beta-galacotsidase staining
1Cardiff School of Biosciences, Cardiff University, Cardiff, Wales, UK.
Methods in Molecular Biology (Clifton, N.J.)
|December 26, 2008
Abstract:
Monitoring the spatial distribution of prespore and pstB cell types is a sensitive method to monitor GSK-3 and Aar activity during Dictyostelium development. Cell-specific expression of lacZ marker genes can be readily detected using enzymatic cleavage of the substrate X-gal. This protocol describes a simple method for beta-galactosidase staining in developed Dictyostelium structures.

