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Dynamics of neutrophilic NF-kB translocation in relation to IL-8 mRNA expression after major trauma

J C Stegmaier1, C Kirchhoff, V Bogner

  • 1Department of Traumatology and Orthopedic Surgery, Ludwig-Maximilians-University Munich, Munich, Germany. julia.stegmaier@med.uni-muenchen.de

Inflammation Research : Official Journal of the European Histamine Research Society ... [Et Al.]
|December 26, 2008
PubMed
Abstract

Insights

Polytrauma patients show an early increase in NF-kB activity and IL-8 mRNA expression in neutrophils, indicating innate immune system down-regulation. This early response precedes later immune system changes.

Area of Science:

  • Immunology
  • Trauma Research

Background:

  • Systemic inflammation post-polytrauma involves neutrophil (PMN) dysregulation.
  • Reduced nuclear factor-kappa B (NF-kB) translocation and cytokine expression characterize this dysregulation.
  • The dynamics of NF-kB activation and IL-8 expression in PMN after major trauma are not fully understood.

Purpose of the Study:

  • To investigate NF-kB nuclear translocation in relation to IL-8 mRNA expression in neutrophils following major trauma.
  • To analyze the temporal dynamics of these markers in the early post-traumatic period.

Main Methods:

  • Blood samples from 15 major trauma patients (NISS > 16) were collected at multiple time points post-trauma.
  • NF-kB translocation was measured using Electrophoretic Mobility Shift Assay (EMSA).
  • IL-8 mRNA expression was quantified by RT-PCR.

Main Results:

  • An initial significant increase in NF-kB translocation and IL-8 mRNA expression was observed in polytrauma patients.
  • NF-kB translocation dynamics differed between survivors and non-survivors.
  • A secondary increase in NF-kB activity and IL-8 expression occurred in survivors after 24 hours.

Conclusions:

  • An early, concomitant increase in NF-kB activity and IL-8 mRNA expression occurs in the post-trauma period.
  • This early increase precedes the down-regulation of the innate immune system.
  • Findings highlight the complex early immune response to major trauma.

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