Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Automated Microbial Diagnostics

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

Methods to Assess Microbial Populations

Methods to Assess Microbial Populations

Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a visible...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Contrasting pollution sensitivity for temperate reef assemblages revealed by microhabitat-resolved eDNA, algal sampling and visual surveys.

Marine pollution bulletin·2026

Same author

Comparison of eDNA with conventional infauna sampling when assessing pollution impacts.

Marine pollution bulletin·2025

Same author

Characterizing organisms from three domains of life with universal primers from throughout the global ocean.

Scientific data·2025

Same author

A marine heatwave drives significant shifts in pelagic microbiology.

Communications biology·2024

Same author

Biogeographical and seasonal dynamics of the marine Roseobacter community and ecological links to DMSP-producing phytoplankton.

ISME communications·2023

Same author

Defining marine bacterioplankton community assembly rules by contrasting the importance of environmental determinants and biotic interactions.

Environmental microbiology·2023

Search research articles

Related Experiment Video

Updated: Jun 26, 2026

A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays

A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays

Published on: August 26, 2013

Evaluation of substrate performance for a microbial diagnostic microarray using a four parameter ranking.

Ursula Sauer¹, Levente Bodrossy, Claudia Preininger

¹Austrian Research Centers GmbH, Seibersdorf, Austria. ursula.sauer@arcs.ac.at

Analytica Chimica Acta

|December 27, 2008

Summary

Choosing the right substrate is crucial for microbial diagnostic microarrays. Several epoxy substrates performed comparably to the reference, offering reliable probe immobilization for gene analysis.

More Related Videos

High-throughput Identification of Bacteria Repellent Polymers for Medical Devices

High-throughput Identification of Bacteria Repellent Polymers for Medical Devices

Published on: November 5, 2016

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Published on: March 3, 2015

Related Experiment Videos

Last Updated: Jun 26, 2026

A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays

A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays

Published on: August 26, 2013

High-throughput Identification of Bacteria Repellent Polymers for Medical Devices

High-throughput Identification of Bacteria Repellent Polymers for Medical Devices

Published on: November 5, 2016

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Published on: March 3, 2015

Area of Science:

Environmental microbiology
Molecular diagnostics
Biotechnology

Background:

Microbial diagnostic microarrays are essential tools for analyzing microbial communities.
Effective probe immobilization on microarray substrates is critical for assay performance.
Various surface chemistries exist for probe binding, impacting microarray reliability.

Purpose of the Study:

To evaluate and compare the performance of different substrates for probe immobilization in microbial diagnostic microarrays.
To assess the impact of substrate surface chemistry on assay specificity, signal, and precision.

Main Methods:

A microbial diagnostic microarray targeting pmoA genes was developed.
Proprietary and competitor epoxy substrates, a 3D substrate, and oligoprobe substrates were tested.
Performance was evaluated based on specificity, signal intensity, inter- and intra-slide precision, using a Cel silylated aldehyde (CSS) slide as a reference.

Main Results:

Four epoxy substrates (ARChip Epoxy, Cel Epoxy, Corning Epoxy, sciChip) and Spot On slides demonstrated satisfactory performance.
These substrates were comparable to the CSS reference slide.
Achieved inter-slide precision ranged from 8% to 18% coefficient of variation (CV), with intra-slide precision below 30%.

Conclusions:

Epoxy-based substrates show significant promise for microbial diagnostic microarray applications.
The evaluated substrates offer reliable probe immobilization and consistent assay performance.
A four-parameter ranking system provides valuable insights into material and protocol performance for microarray development.