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Updated: Jun 26, 2026
A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
Published on: June 10, 2022
On-line coupled dynamic sonication-assisted extraction-liquid chromatography for the determination of phenolic acids
Maarit Kivilompolo1, Tuulia Hyötyläinen
1Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, P.O. Box 55, FIN-00014, Helsinki, Finland.
Abstract:
A fast and efficient on-line coupled dynamic sonication-assisted extraction-liquid chromatography (DSAE-LC) method was developed for the determination of phenolic acids in basil, oregano, rosemary, sage, spearmint and thyme. The extraction and chromatography were coupled via a solid-phase trap filled with strong anion exchange material. The relative standard deviations (RSDs) for the retention times were less than 0.4% and those for the peak heights less than 3% except for gallic acid (RSD 1.2% for the retention times and 11% for the peak heights). Limits of detection were below approximately 3ng.
