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Published on: July 5, 2017
[Separation and purification of proanthocyanidins from rose by macroporous resins]
1College of Pharmaceutical Science, Zhejiang University, Hangzhou 310058, China.
Objective:
To study the separation and purification technology for proanthocyanidins from rose and establish the best operating conditions.
Methods:
Evaluated by static adsorption capacity and elution ratio, five types of macroporous resins including D101, D1300, NKA, AB-8, NKA-II were tested to separate and purify proanthocyanidins. And evaluated by product purity, the concentration of extract sample, pH of extract sample, concentration of eluant and flow rate of elution had been investigated.
Results:
D101 type macroporous resin showed the best property and was suitable for purifying proanthocyanidins from rose. The best operating conditions were as follows: 1.25 mg/ml as the concentration of extract sample, 2 as the pH value of extract sample, 70% ethanol equeous solution as the eluant and 2 ml/min as the flow rate of elution.
Conclusion:
This study can supply a method to separate and purify proanthocyanidins from rose.
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