Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Synthetic Biology02:55

Synthetic Biology

Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Protein Organization01:13

Protein Organization

Overview
Protein Organization01:24

Protein Organization

Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence.
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>De novo</i> grafted coiled-coil peptides as p53/<i>h</i>DM2 inhibitors.

RSC chemical biology·2026
Same author

Enzyme-Free Phosphorylation with Kinetic Gating in a De Novo Coiled-Coil System.

Journal of the American Chemical Society·2026
Same author

Kinesin-1 conformational dynamics are controlled by a cargo-sensitive TPR switch.

eLife·2026
Same author

<i>De novo</i> designed 3-helix bundle peptides and proteins with controlled topology and stability.

Chemical science·2025
Same author

Self-Assembly of Off-Target Peptide Sequences: Implications for the Design of Soft Materials.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Correction to "Functionalized α-Helical Peptide Hydrogels for Neural Tissue Engineering".

ACS biomaterials science & engineering·2025
Same journal

Gas-Responsive Metal-Organic Frameworks for Adaptive Thermal Energy Storage with Tunable Charge-Discharge Temperatures.

Journal of the American Chemical Society·2026
Same journal

Engineering a Thiamine-Dependent Benzoylformate Decarboxylase for Stereodivergent Radical C(sp<sup>3</sup>)-C(sp<sup>3</sup>) Bond Formation.

Journal of the American Chemical Society·2026
Same journal

Accelerated Directional Proton-Coupled Electron Transfer Enabled by Intrinsic Dipole Field in Biomimetic α-Helical Structure.

Journal of the American Chemical Society·2026
Same journal

Alternating Current-Driven Hydrogen Isotope Labeling of Aliphatic Amines Using 1,3-Propanedithiol as an Efficient Hydrogen Atom Transfer Reagent.

Journal of the American Chemical Society·2026
Same journal

Two-Dimensional van der Waals Polar Metal MoOBr<sub>2</sub>.

Journal of the American Chemical Society·2026
Same journal

Negatively Curved Chiral Bilayer Nanographene.

Journal of the American Chemical Society·2026
See all related articles

Related Experiment Video

Updated: Jun 26, 2026

Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins
05:08

Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins

Published on: July 8, 2025

Designed alpha-helical tectons for constructing multicomponent synthetic biological systems.

Elizabeth H C Bromley1, Richard B Sessions, Andrew R Thomson

  • 1School of Chemistry, University of Bristol, Cantock's Close, Bristol, BS8 1TS, UK. Beth.Bromley@bristol.ac.uk

Journal of the American Chemical Society
|January 1, 2009
PubMed
Summary
This summary is machine-generated.

Scientists designed programmed peptide building blocks using a novel algorithm. These peptides self-assemble into specific nanoscale structures, enabling the creation of new synthetic-biological systems.

More Related Videos

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

Related Experiment Videos

Last Updated: Jun 26, 2026

Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins
05:08

Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins

Published on: July 8, 2025

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

Area of Science:

  • Synthetic biology
  • Nanotechnology
  • Biochemistry

Background:

  • Developing synthetic-biological systems requires precise control over nanoscale assembly.
  • Peptide-based building blocks offer a route to programmable nanoscale construction.
  • Existing methods lack specificity in directing peptide self-assembly.

Purpose of the Study:

  • To develop an algorithm for designing peptide building blocks with predictable self-assembly properties.
  • To demonstrate the ability to create specific nanoscale structures using programmed peptides.
  • To validate targeted peptide-peptide interactions in complex mixtures.

Main Methods:

  • An algorithm based on the coiled-coil protein-folding motif was developed to design peptides.
  • Six peptides were synthesized based on the algorithm's design.
  • Peptide self-assembly was analyzed to confirm targeted heterodimer formation and larger structure assembly.

Main Results:

  • The algorithm successfully designed six peptides that form three specific parallel, blunted-ended heterodimers.
  • The designed peptides preferentially formed target heterodimers over homodimers or other configurations.
  • Linked peptides assembled into larger, defined nanoscale rods, confirming predictable assembly.

Conclusions:

  • A computational algorithm can effectively design peptide building blocks for programmable nanoscale assembly.
  • Targeted peptide-peptide interactions can be precisely specified, even in complex mixtures.
  • This approach facilitates the construction of novel synthetic-biological systems and nanostructures.