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Transforming growth factor-beta1 shows an incremental osteoinductive dose-response relationship
J Paul Moxham1, Kevin K Wong, Douglas J Kibblewhite
1Department of Surgery, University of British Columbia, Vancouver, Canada. pmoxham@cw.bc.ca
Objective:
To determine if there is a dose-response curve for TGF-beta1 in a rabbit calvarial defect model.
Study Design:
Controlled animal study using arms of increasing concentrations of TGF-beta1 to evaluate the osteoinductive potential of each arm.
Methods:
Sixteen skeletally mature New Zealand white rabbits were randomized into control and experimental arms. Incremental doses of TGF-beta1 delivered in an inactivated guanidine-extracted demineralized bone matrix (Gu-DBM) carrier were implanted into a critically sized calvarial defect. The animals were sacrificed at 4 weeks and histomorphometric analysis was then accomplished.
Results:
TGF-beta1 showed a dose-response relationship, with the higher doses chosen for this study causing more robust osteoinduction.
Conclusions:
In this pilot dosing study, TGF-beta1 demonstrates increasing osteoinduction with increasing dose levels in this animal model.
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