Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fast heme release from inactive proteins.

J Paul1, M L Smith, K G Paul

  • 1KTH/Royal Institute of Technology, Stockholm, Sweden.

Biochimica Et Biophysica Acta
|September 20, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Prospective Randomised Phase III Clinical Trial Testing the Role of Prophylactic Cranial Radiotherapy in Patients Treated with Trastuzumab for Metastatic Breast Cancer - Anglo Celtic VII.

Clinical oncology (Royal College of Radiologists (Great Britain))·2015
Same author

Estimation of liver iron concentration by dual energy CT images: influence of X-ray energy on sensitivity.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2015
Same author

Exploring two-dimensional electron gases with two-dimensional Fourier transform spectroscopy.

The Journal of chemical physics·2014
Same author

[Association between Hindfoot Alignment and Localisation of Osteochondral Lesions of the Talus].

Zeitschrift fur Orthopadie und Unfallchirurgie·2014
Same author

[Proposed global treatment algorithm for hallux rigidus according to evidence-based medicine].

Revista espanola de cirugia ortopedica y traumatologia·2014
Same author

Feasibility study of a randomised controlled trial to compare (deferred) androgen deprivation therapy and cryotherapy in men with localised radiation-recurrent prostate cancer.

British journal of cancer·2014
Same journal

Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Molecular Basis of Disease Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

General Subjects Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Erratum to 'on the role of exchangeable hydrogen bonds for the kinetics of P680<sup>+·</sup> Q<sub>A</sub> <sup>-·</sup> formation and P680<sup>+·</sup> Pheo<sup>-·</sup> recombination in photosystem II' [Biochim. Biophys. Acta 1276 (1996) 35-44].

Biochimica et biophysica acta·2019
Same journal

Oligomeric state of the light-harvesting complexes B800-850 and B875 from purple bacterium Rubrivivax gelatinosus in detergent solution.

Biochimica et biophysica acta·2019
Same journal

Regulation of pigment content and enzyme activity in the cyanobacterium Nostoc sp. Mac grown in continuous light, a light-dark photoperiod, or darkness.

Biochimica et biophysica acta·2019
See all related articles

The study reveals hemoprotein dissociation kinetics are biphasic. The fast phase indicates an inactive protein form, and the fast-to-slow ratio monitors sample quality for hemoproteins.

Area of Science:

  • Biochemistry
  • Protein Chemistry
  • Spectroscopy

Background:

  • Hemoproteins are crucial biological molecules containing a heme prosthetic group.
  • The dissociation of heme from proteins is a complex process.
  • Previous studies suggested the slow phase of dissociation reflects active protein.

Purpose of the Study:

  • To investigate the kinetics of prosthetic group release from hemoproteins.
  • To re-evaluate the interpretation of biphasic heme-protein dissociation.
  • To establish a reliable method for assessing hemoprotein sample quality.

Main Methods:

  • Analysis of porphyrin release kinetics from hemoproteins.
  • Characterization of biphasic dissociation reactions.
  • Spectroscopic measurements to monitor absorbance changes over time.

Related Experiment Videos

Main Results:

  • Heme-protein separation exhibits a biphasic reaction with distinct fast and slow phases.
  • The initial fast phase of porphyrin release corresponds to an inactive protein conformation.
  • The ratio of fast to slow phase absorbance serves as a sensitive indicator of hemoprotein sample integrity.

Conclusions:

  • The fast phase of dissociation represents an inactive hemoprotein form, contrary to previous assumptions.
  • The fast-to-slow phase ratio is a valuable metric for evaluating hemoprotein sample quality.
  • Extrapolation of the slow phase absorbance allows for accurate determination of unperturbed protein physical parameters.