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Applicability of common methods for short time erosion analysis in vitro
Christian Hannig1, Klaus Becker, Valerie Estelle Yankeu-Ngalene
1Department of Operative Dentistry and Periodontology, University of Freiburg, Hugstetter Strasse 55, D-79106 Freiburg, Germany. Christian.hannig@uniklinik-freiburg.de
Oral Health & Preventive Dentistry
|January 6, 2009
Summary
The colorimetric assay precisely measures short-term dental erosion effects within 5 seconds. This method is superior to diamond indentation and profilometry for quantifying initial erosive damage.
Area of Science:
- Dental research
- Materials science
- Biochemistry
Background:
- Dental erosion is a significant concern, and accurate measurement of early effects is crucial.
- Various methods exist for quantifying dental erosion, but their sensitivity to initial stages varies.
Purpose of the Study:
- To evaluate the applicability of common methods for determining initial dental erosive effects.
- To compare the sensitivity and precision of Knoop's diamond indentation, profilometry, and a colorimetric assay.
Main Methods:
- Enamel surfaces were subjected to in vitro erosion using hydrochloric acid at different pH levels and durations.
- Erosive effects were measured using Knoop's diamond indentation, profilometry, and a colorimetric assay for dissolved calcium ions.
Main Results:
- Profilometry and Knoop's indentation showed limitations in measuring early erosion (e.g., >1 micrometer loss or plateauing measurements) with high variability.
- The colorimetric assay accurately quantified short-term erosive effects within 5 seconds, detecting small amounts of calcium ions with low variability.
Conclusions:
- The colorimetric assay is the superior method for evaluating and quantifying short-term dental erosive effects.
- Diamond indentation and profilometry are less suitable for assessing initial or rapid erosive changes.
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