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Updated: Jun 26, 2026

AQRNA-seq for Quantifying Small RNAs
Published on: February 2, 2024
LNA-modified primers drastically improve hybridization to target RNA and reverse transcription
Agata Fratczak1, Ryszard Kierzek, Elzbieta Kierzek
1Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.
Abstract:
Knowledge about the structure of RNA is crucial to understanding its biological activities. Very often, the presence of unusually thermodynamically stable structural fragments in RNAs, such as hairpins, makes it impossible to apply primer extension to visualize the results of chemical mapping experiments. However, replacement of DNA primers with LNA-modified primers overcomes this limitation. This approach was tested successfully on regulatory OxyS RNA and DsrA RNA from Escherichia coli.
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