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Updated: Jun 26, 2026
Lumican Extraction from Amniotic Membrane and Determination of its Storage Temperature
Published on: October 14, 2022
Immunosuppressive property of dried human amniotic membrane
Choul Yong Park1, Sahar Kohanim, Lei Zhu
1Department of Ophthalmology, Dongguk University, Koyang, South Korea.
Purpose:
To report the immunosuppressive property of dry human amniotic membrane (dHAM).
Methods:
Mouse splenocytes harvested from Balb/c mice were stimulated using functional-grade anti-CD3e antibodies for 4 days either with or without either of 2 commercial types of dHAM (Ambiodry 1 & 2, IOP Inc., Costa Mesa, Calif., USA) added to the culture media. The cell proliferation assay was performed to analyze the extent of splenocyte proliferation.
Results:
dHAMs significantly suppressed mouse splenocyte proliferation compared to control. The suppression by dHAM with intact amniotic epithelium (Ambiodry 2) was significantly stronger than dHAM without epithelium (Ambiodry 1).
Conclusion:
The immunosuppressive property of dHAM was demonstrated using an alloreactive splenocyte proliferation assay.
