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Oxidative DNA damage in retinopathy of prematurity

O Ates1, H H Alp, I Caner

  • 1Department of Ophthalmology Medical Faculty, Ataturk University, Erzurum, Turkey. orhanates@atauni.edu.tr

European Journal of Ophthalmology
|January 6, 2009
PubMed

Insights

Oxidative damage markers, 8-hydroxy 2-deoxyguanosine (8-OHdG) and malondialdehyde (MDA), are elevated in premature infants with retinopathy of prematurity (ROP). These biomarkers show potential for ROP screening in high-risk infants.

Area of Science:

  • Biochemistry
  • Neonatology
  • Ophthalmology

Background:

  • Retinopathy of prematurity (ROP) is a significant cause of visual impairment in premature infants.
  • Oxidative stress is implicated in the pathogenesis of ROP, but specific biomarkers require validation.

Purpose of the Study:

  • To investigate levels of oxidative damage markers, specifically 8-hydroxy 2-deoxyguanosine (8-OHdG) and malondialdehyde (MDA), in premature infants with and without ROP.

Main Methods:

  • Fifty premature infants (birthweight <1500g or gestational age <32 weeks) were recruited and divided into ROP (n=25) and no-ROP (n=25) groups.
  • Blood and urine samples were analyzed for 8-OHdG and MDA levels at 4-6 postnatal weeks.

Main Results:

  • Significantly higher leukocyte and urine 8-OHdG levels were found in infants with ROP compared to controls (p<0.001).
  • Plasma and urine MDA levels were also significantly elevated in the ROP group (p<0.001).
  • Strong correlations were observed between 8-OHdG and MDA levels.

Conclusions:

  • Leukocyte DNA 8-OHdG and urine 8-OHdG levels are potential biomarkers for ROP screening in premature infants.
  • Elevated oxidative damage markers may indicate increased risk or presence of ROP.
Abstract

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