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Updated: Jun 26, 2026

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
Published on: February 1, 2018
Laboratory detection of extended-spectrum beta-lactamase by an automated system
Carlo Gagliotti1, Mario Sarti, Franca Benini
1Area di Programma Rischio Infettivo, Agenzia Sanitaria e Sociale Regionale Emilia-Romagna, Viale Aldo Moro, 21-40127 Bologna, Italy. cgagliotti@regione.emilia-romagna.it
Abstract:
This study aims to evaluate the positive predictive value (PPV) and the negative predictive value (NPV) of Vitek2 in detecting extended-spectrum beta-lactamase (ESBL) phenotypes when compared to a manual confirmatory test as gold standard. A sample of Escherichia coli, Klebsiella spp and Proteus mirabilis isolates were collected by 5 laboratories in the Emilia-Romagna Region (Italy). Vitek2 appears to be an accurate tool to detect ESBL phenotypes of E. coli isolates; some concern remains about its performance with the other bacterial species, especially P. mirabilis.
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