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Induction of circulating neonatal stem cell populations.
N Dainiak1, M Sanders, S Sorba
1Department of Medicine, University of Connecticut Health Center, Farmington 06030.
Blood Cells
|January 1, 1991
Summary
Insulin-like growth factors (IGFs) influence red blood cell production. IGF-I and IGF-II differentially regulate erythropoiesis in adult versus neonatal progenitor cells, suggesting a role in developmental hematopoiesis.
Area of Science:
- Hematology
- Endocrinology
- Developmental Biology
Background:
- Hematopoietic cell growth and differentiation are regulated by paracrine factors, including insulin and insulin-like growth factors (IGFs).
- Previous studies reported IGF-I and IGF-II stimulation of erythropoiesis in adult bone marrow, peripheral blood, and murine fetal liver cells.
Purpose of the Study:
- To investigate the effects of IGF-I and IGF-II on the differentiation and proliferation of human neonatal progenitor cells.
- To compare the influence of IGFs on neonatal versus adult hematopoietic progenitor cells.
Main Methods:
- Cultured human umbilical cord blood, adult blood, and bone marrow cells in a serum-substituted system.
- Assessed the effects of varying concentrations of IGF-I and IGF-II on colony-forming unit-erythroid (CFU-E) proliferation.
- Evaluated responses in both erythropoietin-dependent and -independent progenitor subpopulations.
Main Results:
- IGF-I significantly stimulated adult CFU-E colony formation (up to 265%) and IGF-II (up to 100%) in the presence of erythropoietin.
- IGF-I stimulated erythropoietin-independent adult CFU-Es, while IGF-II did not.
- IGF-II showed a greater stimulatory effect on neonatal CFU-E proliferation than IGF-I, including on erythropoietin-independent cells.
Conclusions:
- Somatomedins (IGFs) are involved in the developmental regulation of erythropoiesis.
- There are distinct differences in IGF responsiveness between adult and neonatal erythroid progenitor cells.