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Updated: Jun 26, 2026

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale
Published on: April 19, 2021
Suppressing one-bond correlations in HMBC spectra: improved performance for the BIRD-HMBC pulse sequence
Julien Furrer1, Damien Thévenet
1Service Analytique Facultaire, Institut de Chimie, Université de Neuchâtel, Rue Emile Argand 11, Case Postale 158, CH-2009 Neuchâtel, Switzerland. julien.furrer@unine.ch
Abstract:
An improved version of the BIRD-HMBC experiment is proposed. In comparison to the original version, the filtering (suppression of (1) J(CH) signals) is accomplished using a double tuned G-BIRD filter positioned in the middle of the long-range correlations evolution period. Compensation of offset dependence by replacing the rectangular 180 degree pulses with the broadband inversion pulses (BIPs), with superior inversion performance and improved tolerance to B(1) field inhomogeneity, significantly improves the sensitivity of the original BIRD-HMBC experiment. For usual one-bond coupling constants ranges (115-180 Hz), optimal results are easily obtained by adjusting the delays, delta, of the BIRD elements to an average J value. For larger ranges (e.g. 110-260 Hz), the use of a double tuned G-BIRD filter allows excellent suppression degrees for all types of one-bond constants present in a molecule, superior to the original scheme and other purging schemes. These attributes make the improved version of the BIRD-HMBC experiment a valuable and robust tool for rapid spectral analysis and rapid checks of molecular skeletons with a minimum spectrometer time.
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