Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 26, 2026

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
09:51

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

Self-loading and cell culture in one layer microfluidic devices.

Li Wang1, Xiao-Fang Ni, Chun-Xiong Luo

  • 1Ecole Normale Supérieure, CNRS UMR 8640, 24 rue Lhomond, 75231, Paris, France.

Biomedical Microdevices
|January 9, 2009
PubMed
Summary

Researchers developed a simple microfluidic device for self-loading and culturing mammalian cells. This method enables high-throughput screening by allowing cells to load and proliferate within specialized chambers.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Triplet-Mediated Photon Upconversion via Near-Infrared-II Excitation without Tetracene Derivatives.

Journal of the American Chemical Society·2026
Same author

Programmable In Vivo Synthesis of Quantum Dots.

Angewandte Chemie (International ed. in English)·2026
Same author

Beyond-1000 nm low-energy sunlight-driven photocatalysis enabled by quantum dot-based photon upconversion.

National science review·2026
Same author

Simultaneous Localization of Electrons/Holes by Surface Cationic Dangling-Bonds/Vacancies for Synergistically Boosting Photocatalytic Activity.

Journal of the American Chemical Society·2026
Same author

Efficient near-infrared-excitable quantum dot-based triplet-triplet annihilation upconversion with a record anti-Stokes shift <i>via</i> low coverage of mono-styryl-BODIPY ligands.

Chemical science·2026
Same author

Rapid Discrimination of Single-Virus Entry Pathways via a Bioorthogonal Activatable Fluorescent Probe.

Angewandte Chemie (International ed. in English)·2026

Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell Biology

Background:

  • Microfluidic devices are crucial for high-throughput screening.
  • Efficient cell loading and culture are essential for reproducible results.
  • Current methods often require complex procedures for cell handling.

Purpose of the Study:

  • To develop a simple, self-loading microfluidic system for mammalian cell culture.
  • To investigate the relationship between chamber geometry and cell loading efficiency.
  • To demonstrate the feasibility of long-term cell proliferation monitoring in micro-chambers.

Main Methods:

  • Fabrication of a polydimethylsiloxane (PDMS) microfluidic device using one-layer soft lithography and thermal bonding.
  • Degassing the PDMS device to enable self-loading of cell suspensions.

More Related Videos

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip
07:05

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip

Published on: September 27, 2019

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays
18:11

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Published on: October 1, 2007

Related Experiment Videos

Last Updated: Jun 26, 2026

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
09:51

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip
07:05

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip

Published on: September 27, 2019

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays
18:11

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Published on: October 1, 2007

  • Analysis of cell loading efficiency and proliferation in triangular chambers of varying sizes.
  • Long-term monitoring of HeLa cell growth over 11 days.

    • Main Results:

    • Self-loading of mammalian cells was achieved after 30 minutes of PDMS degassing.
    • Cell loading efficiency demonstrated a clear dependence on chamber size, with smaller chambers enabling single-cell loading.
    • Cell proliferation led to heterogeneous area density in larger chambers.
    • Complete chamber occupancy was achieved in the largest chambers within 11 days.

    Conclusions:

    • The developed microfluidic device offers a simple and effective method for self-loading and culturing mammalian cells.
    • The system is suitable for high-throughput screening applications.
    • Chamber geometry significantly influences cell loading and distribution, allowing for tailored experimental designs.