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Adsorption Isotherms II

Adsorption Isotherms II

Brunauer, Emmett, and Teller (BET) introduced a theory in 1938 that modified Langmuir's assumptions to explain multilayer physical adsorption. This theory is applicable to Type II isotherms and provides a more realistic picture of adsorption processes. The BET theory assumes a uniform solid surface with localized adsorption sites, where adsorption at one site doesn't affect adsorption at neighboring sites. This theory also allows for the possibility of additional molecules being adsorbed on top...

Analyte Adsorption and Distribution

Analyte Adsorption and Distribution

In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and solvents...

Adsorption of Gases on Solids

Adsorption of Gases on Solids

Adsorption is a process where molecules, known as the adsorbates, accumulate on a surface, which is referred to as the adsorbent or substrate. Occurring at the solid-gas interface, this phenomenon is crucial in various scientific and industrial contexts. The reverse of adsorption is desorption.Two types of adsorptions exist: physical (physisorption) and chemical (chemisorption). Physisorption involves gas molecules held to the solid's surface by relatively weak intermolecular van der Waals...

Adsorption Isotherms I

Adsorption Isotherms I

Adsorption isotherms are mathematical models that describe how molecules in a gas or liquid phase interact with surfaces. Two of the most common isotherm models are the Langmuir and Freundlich isotherms, which relate to Type I monolayer chemisorption. The Langmuir model is based on four key assumptions:• Adsorption cannot exceed monolayer coverage.• All surface sites are equivalent.• Molecules adsorb only at vacant sites.• There are no interactions between adsorbed molecules.Consider the...

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Related Experiment Video

Updated: Jun 26, 2026

Monitoring Protein Adsorption with Solid-state Nanopores

Monitoring Protein Adsorption with Solid-state Nanopores

Published on: December 2, 2011

Single particle adsorbing transfer system.

Daniela Woide¹, Veronika Mayer, Thorsten Wachtmeister

¹Helmholtz Zentrum Munich, German Research Center for Environmental Health, Institute of Radiation Protection, Ingolstädter Landstrasse 1, 85764, Neuherberg, Germany.

Biomedical Microdevices

|January 9, 2009

Summary

A new laser microdissection technique enables precise, contamination-free horizontal transfer of single particles like cells or chromosomes. This method is crucial for advanced lab-on-a-chip devices and single-cell analyses.

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Published on: May 22, 2020

Area of Science:

Biotechnology
Microfluidics
Cell Biology

Background:

Laser microdissection (LMD) is a powerful technique for isolating specific biological materials.
Current methods for transferring microdissected samples can be inefficient and prone to contamination.
Advancements are needed for precise sample handling in miniaturized analytical systems.

Purpose of the Study:

To develop a novel, highly selective, and gentle method for horizontal transfer of single particles post-laser microdissection.
To enable precise positioning and contamination-free transfer of microdissected biological materials onto microchip devices.

Main Methods:

A single particle adsorbing system utilizing low-pressure technology was developed.
The system allows for controlled horizontal transfer and precise sample release.
Target positions on planar microchip devices can be predefined.

Main Results:

The technique successfully achieved highly selective and gentle horizontal transfer of microdissected fixed and living materials.
Contamination-free transfer of isolated materials, including single cells and chromosomes, was demonstrated.
Precise positioning and release of samples onto microchip devices were achieved.

Conclusions:

The developed method offers a significant advancement for precise sample manipulation in microfluidic applications.
This technique is indispensable for novel lab-on-a-chip systems, particularly for nanoscale polymerase chain reaction (PCR) and single-cell analysis.
The ability to transfer minute amounts of material, such as a single cell, enables immediate downstream analysis.