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Updated: Jun 26, 2026

Mouse Models Of Helicobacter Infection And Gastric Pathologies
Published on: October 18, 2018
Acute effects of Helicobacter pylori extracts on gastric mucosal blood flow in the mouse
Johanna Henriksnäs1, Christer Atuma, Mia Phillipson
1Department of Medical Cell Biology, Uppsala University, PO Box 571, SE-751 23 Uppsala, Sweden. johanna.henriksnas@mcb.uu.se
Aim:
To investigate the mechanisms underlying the reduction in gastric blood flow induced by a luminal water extract of Helicobacter pylori (HPE).
Methods:
The stomachs of isoflurane-anesthetized mice were exteriorized, and the mucosal surface exposed. Blood flow was measured with the laser-Doppler technique, and systemic arterial blood pressure monitored. C57BL/6 mice were exposed to water extract produced from H pylori strain 88-23. To investigate the role of a nerve- or iNOS-mediated pathway, we used intraluminal lidocaine and iNOS-/- mice. Blood flow response to the endogenous nitric oxide synthase inhibitor asymmetric dimethyl arginine (ADMA) was also assessed.
Results:
In wild-type mice, HPE decreased mucosal blood flow by approximately 30%. This reduction was abolished in iNOS-deficient mice, and by pre-treatment with lidocaine. Luminally applied ADMA resulted in reduction in blood flow similar to that observed in wild-type mice exposed to HPE.
Conclusion:
A H pylori water extract reduces gastric mucosal blood flow acutely through iNOS- and nerve-mediated pathways.
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