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Updated: Jun 24, 2026

Soil Lysimeter Excavation for Coupled Hydrological, Geochemical, and Microbiological Investigations
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Soil Lysimeter Excavation for Coupled Hydrological, Geochemical, and Microbiological Investigations

Published on: September 11, 2016

Developing and testing a diagnostic probe for grape phylloxera applicable to soil samples.

Karen Herbert1, Kevin Powell, Alan Mckay

  • 1CRC for Viticulture, P.O. Box 154, Glen Osmond, SA 5064, Australia.

Journal of Economic Entomology
|January 13, 2009
PubMed
Summary
This summary is machine-generated.

A new DNA test accurately detects grape phylloxera (Daktulosphaira vitifoliae) in soil, offering a more sensitive method than traditional surveys. This advancement aids in early pest management for grapevines (Vitis spp.).

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Area of Science:

  • Agricultural Entomology
  • Molecular Diagnostics
  • Viticulture

Background:

  • Grape phylloxera (Daktulosphaira vitifoliae) is a significant global pest of grapevines (Vitis spp.).
  • Effective management relies on early detection of phylloxera infestations.
  • Current detection methods, like ground surveys, have limitations in sensitivity and scope.

Purpose of the Study:

  • To develop and validate a sensitive DNA-based assay for detecting grape phylloxera in soil.
  • To compare the efficacy of the DNA assay against standard ground surveys.
  • To establish a quantitative method for assessing phylloxera infestation levels.

Main Methods:

  • Development of species-specific primers targeting the internal transcribed spacer region 2 of grape phylloxera.
  • Confirmation of primer specificity against various vineyard organisms and aphid genera.
  • Conversion of the assay to a real-time polymerase chain reaction (TaqMan MGB) format.
  • DNA extraction from soil samples and subsequent PCR analysis.
  • Field validation at a known grape phylloxera infestation site in the Yarra Valley, Victoria, Australia.

Main Results:

  • The developed DNA assay demonstrated high sensitivity in detecting grape phylloxera crawlers in spiked soil samples.
  • The assay was significantly more sensitive than ground surveys for detecting phylloxera on vine roots in an infested vineyard.
  • The DNA assay provided quantitative data, with DNA concentrations correlating closely with the number of phylloxera crawlers.
  • The method is applicable year-round and has potential for genotype-specific virulence assessment.

Conclusions:

  • The developed soil-based DNA assay is a highly sensitive and quantitative tool for detecting grape phylloxera.
  • This method offers a significant improvement over traditional ground surveys for early pest detection and management.
  • The assay's versatility and potential for further modification make it a valuable asset for viticulture pest control.