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Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria
Published on: September 16, 2013
Alternative procedures for analysis of lipid A modification with phosphoethanolamine or aminoarabinose
1Faculty of Pharmaceutical Sciences, Doshisha Women's College, Kyotanabe, Kyoto 610-0395, Japan. kkawasak@dwc.doshisha.ac.jp <kkawasak@dwc.doshisha.ac.jp>
Journal of Microbiological Methods
|January 13, 2009
Abstract:
MALDI-TOF mass spectrometry analysis of lipid A prepared using a Tri-reagent-based procedure with a 5-chloro-2-mercaptobenzothiazole matrix was preferable for the detection of phosphoethanolamine modification. In contrast, the analysis of lipid A prepared using an LPS extraction kit-based procedure with 2,5-dihydroxybenzoic acid was preferable for the detection of aminoarabinose modification.
