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Updated: Jun 26, 2026

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
Published on: July 12, 2013
Application of silicon nanowires and indium tin oxide surfaces in desorption electrospray ionization
Jaroslav Pól1, Petr Novák, Michael Volný
1Laboratory of Molecular Structure Characterization, Institute of Microbiology, Academy of Sciences of the Czech Republic. jaroslav.pol@helsinki.fi
Abstract:
Two nanostructured surfaces are introduced as advantageous substrates for desorption electrospray ionization mass spectrometry (DESI-MS). Nano-assisted laser desorption/ionization (NALDI) plates coated with silicon nanowires (SiNWs) and indium tin oxide (ITO) layers on glass are both conductive non-polar surfaces that were originally designed as superior substrates for matrix-free laser desorption/ionization. In this study, NALDI/SiNWs and ITO were tested as potentially useful DESI substrates for selected model analytes (cyclosporine, beauverolide, surfactin and nystatin). Both nanostructured surfaces produced more intense and longer-lasting signals than other tested surfaces (polytetrafluoroethylene, glass, polymethylmethacrylate and chromatography paper).
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