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Updated: Jun 26, 2026

Mass Spectrometric Analysis of Glycosphingolipid Antigens
Published on: April 16, 2013
Analysis of complex oligosaccharides using graphitized carbon liquid chromatography/mass spectrometry
Lívia Budai1, Ferenc Pollreisz, Olivér Ozohanics
1Hungarian Academy of Sciences, Chemical Research Center, H-1025, Pusztaszeri 59-67, Budapest, Hungary.
Abstract:
The sugar fraction of alpha-1 acid glycoprotein (AGP) was studied using porous graphitized carbon (PGC) chromatography coupled to mass spectrometry. Electrospray ionization provides efficient control over fragmentation; at low collision energy only molecular species were observed, allowing accurate oligosaccharide profiling. PGC chromatography was useful separating 18 sugars differing in monosaccharide composition. Most of these were separated into several isomeric forms; altogether 49 different oligosaccharides were found in AGP.
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