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Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Related Experiment Video

Updated: Jun 26, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Ultrathin four-reflection imager.

Eric J Tremblay1, Ronald A Stack, Rick L Morrison

  • 1Department of Electrical and Computer Engineering, University of California, San Diego, La Jolla, California 92093, USA. etremblay@ucsd.edu

Applied Optics
|January 13, 2009
PubMed
Summary
This summary is machine-generated.

We developed an ultrathin four-reflection imager with a compact 5.5 mm track length. This novel optical design extends depth of field and corrects chromatic aberration for enhanced imaging performance.

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Area of Science:

  • Optical Engineering
  • Image Sensor Technology
  • Compact Imaging Systems

Background:

  • Traditional imagers face limitations in size and depth of field.
  • Miniaturization is crucial for portable and integrated imaging applications.

Purpose of the Study:

  • To design and demonstrate an ultrathin four-reflection imager.
  • To improve the depth of field and chromatic aberration of the imager.

Main Methods:

  • Utilized a four-reflection optical design for miniaturization.
  • Implemented pupil-phase encoding and postprocessing techniques.
  • Integrated a 1.92-megapixel color image sensor with 3-microm pixels.

Main Results:

  • Achieved a focal length of 18.6 mm within a 5.5 mm track length.
  • Demonstrated a 17-degree field of view.
  • Successfully extended depth of field and compensated axial chromatic aberration.

Conclusions:

  • The ultrathin four-reflection imager offers a compact solution for high-resolution imaging.
  • Pupil-phase encoding is effective for enhancing the performance of compact imagers.