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Related Concept Videos

Satellite Stem Cells and Muscular Dystrophy01:21

Satellite Stem Cells and Muscular Dystrophy

Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
Source And Potency Of Stem Cells01:27

Source And Potency Of Stem Cells

Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...
Formation of Muscle Fibers from Myoblasts01:13

Formation of Muscle Fibers from Myoblasts

De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
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Embryonic Stem Cells00:57

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
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Embryonic Stem Cells00:58

Embryonic Stem Cells

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Multipotency and Niche of Bulge Stem Cell

A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...

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Updated: Jun 26, 2026

Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo
09:19

Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo

Published on: February 15, 2021

Myogenic stem cells.

Anna Burdzińska1, Kamila Gala, Leszek Paczek

  • 1Department of Immunology, Transplantology and Internal Disease, Transplantation Institute, Medical University of Warsaw, Poland. aniaburdzia@interia.pl

Folia Histochemica Et Cytobiologica
|January 15, 2009
PubMed
Summary

Muscle and bone marrow contain myogenic stem cells, with some derived from bone marrow contributing to muscle regeneration. Understanding these cells is key to improving aged muscle repair for cell therapies.

Area of Science:

  • Cell Biology
  • Regenerative Medicine
  • Musculoskeletal Biology

Background:

  • Skeletal muscle harbors heterogeneous myogenic stem cells, including satellite cells and bone marrow-derived progenitors.
  • Bone marrow-derived cells can differentiate into muscle stem cells, potentially expressing Pax7 and adopting satellite cell positions.
  • Mesenchymal stem cells (MSCs) from bone marrow possess myogenic potential, though in vitro differentiation requires optimization.

Purpose of the Study:

  • To investigate the origin and characteristics of myogenic stem cells in skeletal muscle and bone marrow.
  • To explore the contribution of bone marrow-derived cells to muscle regeneration and repair.
  • To understand the factors contributing to impaired regenerative potential in aged skeletal muscle for therapeutic applications.

Main Methods:

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Identification and Analysis of Myogenic Progenitors In Vivo During Acute Skeletal Muscle Injury by High-Dimensional Single-Cell Mass Cytometry

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09:19

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Identification and Analysis of Myogenic Progenitors In Vivo During Acute Skeletal Muscle Injury by High-Dimensional Single-Cell Mass Cytometry
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Identification and Analysis of Myogenic Progenitors In Vivo During Acute Skeletal Muscle Injury by High-Dimensional Single-Cell Mass Cytometry

Published on: December 1, 2023

  • Tracking of bone marrow-derived cells recruited to muscle tissue following injury or exercise.
  • Phenotypic analysis of recruited cells, including expression of specific markers like Pax7.
  • Assessment of myogenic differentiation potential of mesenchymal stem cells (MSCs) in vitro.

Main Results:

  • Bone marrow-derived cells are recruited to muscle and can differentiate into muscle stem cells, acquiring satellite cell characteristics.
  • Mesenchymal stem cells (MSCs) demonstrate myogenic potential, indicating a possible therapeutic avenue.
  • Age-related decline in muscle regeneration appears to be influenced by both extrinsic and intrinsic factors.

Conclusions:

  • Bone marrow is a source of myogenic stem cells that contribute to skeletal muscle regeneration.
  • Optimizing in vitro myogenic differentiation of MSCs is crucial for cell-based therapies.
  • Reversible extrinsic factors play a significant role in age-related muscle degeneration, but intrinsic stem cell properties are also implicated.