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Enzymatic method for measuring ethylene glycol with a centrifugal analyzer
1Department of Pathology, University of New Mexico School of Medicine, Albuquerque 87131.
Abstract:
We describe a semiautomated, enzymatic method of analysis for ethylene glycol in plasma. Glycerol dehydrogenase (EC 1.1.1.6) from Enterobacter aerogenes, which has high specificity for ethylene glycol, is mixed with 3 microL of specimen in a centrifugal analyzer. NAD+ is added to initiate the reaction, and 450 s after an initial 90-s delay, the absorbance at 340 nm is measured. Monthly calibration with a five-point calibration curve is sufficient to provide between-day precision (CV) of 2% to 5%. The detection limit is 0.3 mmol/L with a CV of 10%. There is no significant interference from ethanol, methanol, isopropanol, acetone, lactate, or glycerol.