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Quantification of serum amyloid P by enzyme-linked immunosorbent assay
R Saïle1, A Kandoussi, M Deveaux
1Laboratoire de Recherche Néphrologique, Hôpital Calmette, Lille, France.
Clinical Chemistry
|October 1, 1991
Abstract:
This enzyme-linked immunosorbent assay procedure for quantifying serum amyloid P (SAP) in human plasma makes use of affinity-purified polyclonal antibodies to SAP in a "sandwich"-type format. The procedure is sensitive, reproducible, simple, and easily automatable. Results correlate well with those by a rocket immunoelectrophoresis method performed with the same antibodies. Sera from apparently normal individuals had a mean SAP content of 44.17 mg/L and increased with age.