Related Experiment Video
Updated: Jun 26, 2026
Orthotopic Small Bowel Transplantation in Rats
Published on: November 6, 2012
[Operative techniques and common complications of modified orthotopic intestinal transplantation in rats]
Li-Jun Xue1, Lu Yin, Hui-Jiang Zhou
1Department of Surgery, Ruijin Hospital, Shanghai Institute of Digestive Surgery, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China. yindalu@yahoo.com.cn
Objective:
To investigate operative techniques, treatment and precaution of common complications of orthotopic intestinal transplantation in the rats.
Methods:
Orthotopic intestinal transplantation was performed in 120 rats by modified three cuffs method. The causes, treatment and precaution of common complications were analyzed retrospectively.
Results:
The 7-day survival rate of recipients was 82.5% and the 30-day survival rate was 68.3%. The average volume of bleeding in the recipient operation was less than 1 ml. The result obtained from the above 99 recipients was satisfactory. The main reasons of final failure and death were as follows: anastomotic bleeding(5 rats), portal vein thrombus(2 rats), arterial thrombus(4 rats), air embolism(1 rat), infection of abdominal cavity(4 rats), aspiration pneumonitis (2 rats), anesthetic accident(2 rats) and kinking of graft intestine(1 rat).
Conclusions:
The sophisticated surgical technique and the delicate surgical manipulation are the prerequisite of preventing operational complication. Improving operative techniques and being familiar with the common complications can reduce the occurrence of complications and increase operative successful rate.
